Journal: Materials & Design

Article Title: Bifunctional copper ion/melittin incorporated hydrogel with antimicrobial and antioxidant capabilities for infected skin wound healing

doi: 10.1016/j.matdes.2025.113631

Figure Lengend Snippet: Fig. 10. Immunohistochemical staining of inflammation-related markers of wound healing in SD rats. (a, b) Immunohistochemical staining images of MPO and CD68 proteins. Yellow arrows represent areas of positive immunohistochemical staining in healing skin. (c, d) The statistical analysis results of protein expression in the immunohistochemical staining images. Data are presented as means ± SD (n = 4). One-way ANOVA was used to calculate significant difference, ****p < 0.001.

Article Snippet: Immunohistochemical (IHC) staining was performed for myeloperoxidase (MPO) (Cat#66177–1-Ig, 1:200, Proteintech, China) and CD68 (Cat#66231–2-Ig, 1:200, Proteintech, China).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Stem cell research & therapy

Article Title: Subretinal microglia support donor photoreceptor survival in rd1 mice.

doi: 10.1186/s13287-024-04052-0

Figure Lengend Snippet: Fig. 2 Macrophage and microglia detection after transplantation. A. In rd1 mice, macrophages (CD68+) were observed in the choroid when donor cells either survived (left) or died (middle). In wild-type mice, dead donor cells were engulfed by macrophages (right). Scale bar = 10 μm. B. In trans-scleral injection group, while microglial cells surrounded surviving donor cells in rd1 mice (i), they could hardly be detected around dead cell debris (ii). In wild-type mice, microglia/macrophages engulfed dead donor cells (iii). Following trans-vitreous injection, both dead (arrows) and surviving donor cells (arrowheads) were found in the subretinal space. While macrophages/microglia engulfed the dead cells (arrows), microglia were not close to surviving cells (iv). C. A Comparison of chemokines expression between wild-type and rd1 mice showed a significant increase in the mRNA levels of ccl2, ccl3 and cxcl2 in rd1 mice. D. The comparison of cytokine levels between wild-type and rd1 mice showed no significant difference. E. No significant differences were found in oxidative stress factors between wild-type and rd1 mice. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The following primary antibodies were used in the study: CD68 (1:400,#97778, Cell Signaling Technology), Iba1 (1:200, #019-19741, Wako), Galectin-3 (1:200, #sc-53127, Santa Cruz), F4/80(1:400, #71299, Cell Signaling Technology), CD11b (1:200, 101,209, Biolegend).

Techniques: Transplantation Assay, Injection, Comparison, Expressing

Journal: Alzheimer's research & therapy

Article Title: Targeting early tau pathology: probiotic diet enhances cognitive function and reduces inflammation in a preclinical Alzheimer's model.

doi: 10.1186/s13195-025-01674-1

Figure Lengend Snippet: Fig. 4 The effects of probiotic supplementation on peripheral and central inflammation. A Concentration of IL6 (mg/ml) from plasma at 12-months of age. N: GFP + Ctrl Diet: 4 F/6 M; GFP + P/P Diet: 4 F/6 M; htauE14 + Ctrl Diet: 8 F/2 M; htauE14 + P/P Diet: 6 F/4M. B Concentration of TNFa (mg/ml) from plasma at 12-months of age. N: GFP + Ctrl Diet: 6 F/7 M; GFP + P/P Diet: 6 F/6 M; htauE14 + Ctrl Diet: 9 F/2 M; htauE14 + P/P Diet: 7 F/5M. C Concentration of IL10 (mg/ml) from plasma at 12-months of age. N: GFP + Ctrl Diet: 5 F/3 M; GFP + P/P Diet: 5 F/6 M; htauE14 + Ctrl Diet: 6 F/2 M; htauE14 + P/P Diet: 5 F/4M. D Number of Iba1 cells per mm2 in the LC. N: GFP + Ctrl Diet: 6 F/4 M; GFP + P/P Diet: 5 F/4 M; htauE14 + Ctrl Diet: 4 F/4 M; htauE14 + P/P Diet: 3 F/3M. E Number of CD68 cells per mm2 in the LC. N: GFP + Ctrl Diet: 6 F/4 M; GFP + P/P Diet: 4 F/4 M; htauE14 + Ctrl Diet: 4 F/4 M; htauE14 + P/P Diet: 4 F/4M. F Number of GFAP cells per mm2 in the LC. N: GFP + Ctrl Diet: 3 F/3 M; GFP + P/P Diet: 5 F/4 M; htauE14 + Ctrl Diet: 3 F/3 M; htauE14 + P/P Diet: 3 F/3M. G Mean intensity of Albumin staining in the LC. N: GFP + Ctrl Diet: 4 F/4 M; GFP + P/P Diet: 4 F/4 M; htauE14 + Ctrl Diet: 4 F/4 M; htauE14 + P/P Diet: 5 F/4 M. Example images of Iba-1, CD68, GFAP, and Albumin, respectively, in the LC across all groups were shown in the lower panels. Scale bars: 50 μm. Arrows indicate positively stained cells. *p < 0.05, **p < 0.01

Article Snippet: Primary antibodies used included: dopamine β-hydroxylase (DBH) (MAB308, Millipore-Sigma, 1:2000), Ionized calcium-binding adaptor molecule 1 (Iba1) (019-19741, Wako, 1:2000), Cluster of Differentiation 68 (CD68) (MCA341GA, Bio-Rad, 1:1000), Glial fibrillary acidic protein (GFAP) (MAB3402, MilliporeSigma, 1:500), and Albumin (16475-l-AP, ProteinTech, 1:1000) in PBS with 0.2% Triton-X and 2% goat serum.

Techniques: Concentration Assay, Clinical Proteomics, Staining